In the video titled "IGNORING MARIO FOR 24 HOURS 😨 | Hermanas JM", the content revolves around two sisters, likely named JM, who decide to ignore their brother Mario for a whole day. Throughout the video, they show various ways in which they avoid interacting with Mario, including ignoring his messages and requests for attention. Despite Mario's attempts to get their attention, the sisters continue to ignore him, causing some tension and frustration among them. As the day goes on, Mario becomes increasingly desperate for their acknowledgment, leading to some humorous and entertaining moments. Ultimately, the video highlights the dynamics of sibling relationships and the impact of ignoring someone close to you for an extended period of time.